| Title |
The effect of nicotine on the mechanical properties of mesenchymal stem cells
|
|---|---|
| Published in |
Cell health and cytoskeleton, March 2012
|
| DOI | 10.2147/chc.s24381 |
| Pubmed ID | |
| Authors |
Herman Cheung, Pelaez, Dias, Ziebarth, Herman Cheung |
| Abstract |
PURPOSE: To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. METHODS: MSCs were synchronized to the G(0) stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 µM, 0.5 µM, and 1 µM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. RESULTS: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 µM and 1.0 µM concentrations only. CONCLUSIONS: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | 5% |
| Singapore | 1 | 5% |
| Unknown | 17 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 53% |
| Student > Doctoral Student | 2 | 11% |
| Student > Master | 2 | 11% |
| Professor | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 3 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 21% |
| Biochemistry, Genetics and Molecular Biology | 3 | 16% |
| Medicine and Dentistry | 3 | 16% |
| Engineering | 2 | 11% |
| Social Sciences | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 4 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2012.
All research outputs
#20,110,957
of 25,584,565 outputs
Outputs from Cell health and cytoskeleton
#14
of 20 outputs
Outputs of similar age
#128,591
of 168,428 outputs
Outputs of similar age from Cell health and cytoskeleton
#1
of 1 outputs
Altmetric has tracked 25,584,565 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20 research outputs from this source. They receive a mean Attention Score of 2.9. This one scored the same or higher as 6 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 168,428 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them