| Title |
RGDS-functionalized polyethylene glycol hydrogel-coated magnetic iron oxide nanoparticles enhance specific intracellular uptake by HeLa cells
|
|---|---|
| Published in |
International Journal of Nanomedicine, April 2012
|
| DOI | 10.2147/ijn.s29442 |
| Pubmed ID | |
| Authors |
Caner Nazli, Tugba Ipek Ergenc, Yasemin Yar, Havva Yagci Acar, Seda Kizilel |
| Abstract |
The objective of this study was to develop thin, biocompatible, and biofunctional hydrogel-coated small-sized nanoparticles that exhibit favorable stability, viability, and specific cellular uptake. This article reports the coating of magnetic iron oxide nanoparticles (MIONPs) with covalently cross-linked biofunctional polyethylene glycol (PEG) hydrogel. Silanized MIONPs were derivatized with eosin Y, and the covalently cross-linked biofunctional PEG hydrogel coating was achieved via surface-initiated photopolymerization of PEG diacrylate in aqueous solution. The thickness of the PEG hydrogel coating, between 23 and 126 nm, was tuned with laser exposure time. PEG hydrogel-coated MIONPs were further functionalized with the fibronectin-derived arginine-glycine-aspartic acid-serine (RGDS) sequence, in order to achieve a biofunctional PEG hydrogel layer around the nanoparticles. RGDS-bound PEG hydrogel-coated MIONPs showed a 17-fold higher uptake by the human cervical cancer HeLa cell line than that of amine-coated MIONPs. This novel method allows for the coating of MIONPs with nano-thin biofunctional hydrogel layers that may prevent undesirable cell and protein adhesion and may allow for cellular uptake in target tissues in a specific manner. These findings indicate that the further biofunctional PEG hydrogel coating of MIONPs is a promising platform for enhanced specific cell targeting in biomedical imaging and cancer therapy. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 3% |
| Spain | 1 | 1% |
| Unknown | 68 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 35% |
| Student > Master | 14 | 20% |
| Researcher | 9 | 13% |
| Professor > Associate Professor | 6 | 8% |
| Student > Doctoral Student | 5 | 7% |
| Other | 6 | 8% |
| Unknown | 6 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 17 | 24% |
| Engineering | 14 | 20% |
| Materials Science | 10 | 14% |
| Agricultural and Biological Sciences | 8 | 11% |
| Biochemistry, Genetics and Molecular Biology | 6 | 8% |
| Other | 8 | 11% |
| Unknown | 8 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2014.
All research outputs
#16,188,873
of 25,584,565 outputs
Outputs from International Journal of Nanomedicine
#1,880
of 4,077 outputs
Outputs of similar age
#105,263
of 173,277 outputs
Outputs of similar age from International Journal of Nanomedicine
#43
of 64 outputs
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