| Title |
In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 femtosecond near-infrared laser pulse
|
|---|---|
| Published in |
International Journal of Nanomedicine, May 2012
|
| DOI | 10.2147/ijn.s31768 |
| Pubmed ID | |
| Authors |
Mitsuhiro Terakawa, Yasuyuki Tsunoi, Tatsuki Mitsuhashi |
| Abstract |
Pulsed laser interaction with small metallic and dielectric particles has been receiving attention as a method of drug delivery to many cells. However, most of the particles are attended by many risks, which are mainly dependent upon particle size. Unlike other widely used particles, biodegradable particles have advantages of being broken down and eliminated by innate metabolic processes. In this paper, the perforation of cell membrane by a focused spot with transparent biodegradable microspheres excited by a single 800 nm, 80 fs laser pulse is demonstrated. A polylactic acid (PLA) sphere, a biodegradable polymer, was used. Fluorescein isothiocyanate (FITC)-dextran and short interfering RNA were delivered into many human epithelial carcinoma cells (A431 cells) by applying a single 80 fs laser pulse in the presence of antibody-conjugated PLA microspheres. The focused intensity was also simulated by the three-dimensional finite-difference time-domain method. Perforation by biodegradable spheres compared with other particles has the potential to be a much safer phototherapy and drug delivery method for patients. The present method can open a new avenue, which is considered an efficient adherent for the selective perforation of cells which express the specific antigen on the cell membrane. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 4% |
| Unknown | 24 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 28% |
| Researcher | 6 | 24% |
| Student > Ph. D. Student | 4 | 16% |
| Student > Doctoral Student | 3 | 12% |
| Student > Postgraduate | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 6 | 24% |
| Engineering | 4 | 16% |
| Materials Science | 2 | 8% |
| Agricultural and Biological Sciences | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Other | 6 | 24% |
| Unknown | 3 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2012.
All research outputs
#20,656,820
of 25,374,647 outputs
Outputs from International Journal of Nanomedicine
#3,127
of 4,123 outputs
Outputs of similar age
#137,645
of 175,822 outputs
Outputs of similar age from International Journal of Nanomedicine
#56
of 83 outputs
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