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Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery

Overview of attention for article published in International Journal of Nanomedicine, March 2012
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

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1 X user
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3 patents

Citations

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68 Dimensions

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85 Mendeley
Title
Low molecular weight chitosan nanoparticulate system at low N:P ratio for nontoxic polynucleotide delivery
Published in
International Journal of Nanomedicine, March 2012
DOI 10.2147/ijn.s26571
Pubmed ID
Authors

Mohamad Alameh, Diogo DeJesus, Myriam Jean, Vincent Darras, Marc Thibault, Marc Lavertu, Michael D Buschmann, Abderrazzak Merzouki

Abstract

Chitosan, a natural polymer, is a promising system for the therapeutic delivery of both plasmid DNA and synthetic small interfering RNA. Reports attempting to identify the optimal parameters of chitosan for synthetic small interfering RNA delivery were inconclusive with high molecular weight at high amine-to-phosphate (N:P) ratios apparently required for efficient transfection. Here we show, for the first time, that low molecular weight chitosan (LMW-CS) formulations at low N:P ratios are suitable for the in vitro delivery of small interfering RNA. LMW-CS nanoparticles at low N:P ratios were positively charged (ζ-potential ~20 mV) with an average size below 100 nm as demonstrated by dynamic light scattering and environmental scanning electron microscopy, respectively. Nanoparticles were spherical, a shape promoting decreased cytotoxicity and enhanced cellular uptake. Nanoparticle stability was effective for at least 20 hours at N:P ratios above two in a slightly acidic pH of 6.5. At a higher basic pH of 8, these nanoparticles were unravelled due to chitosan neutralization, exposing their polynucleotide cargo. Cellular uptake ranged from 50% to 95% in six different cell lines as measured by cytometry. Increasing chitosan molecular weight improved nanoparticle stability as well as the ability of nanoparticles to protect the oligonucleotide cargo from nucleases at supraphysiological concentrations. The highest knockdown efficiency was obtained with the specific formulation 92-10-5 that combines sufficient nuclease protection with effective intracellular release. This system attained >70% knockdown of the messenger RNA, similar to commercially available lipoplexes, without apparent cytotoxicity. Contrary to previous reports, our data demonstrate that LMW-CS at low N:P ratios are efficient and nontoxic polynucleotide delivery systems capable of transfecting a plethora of cell lines.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 1%
Ireland 1 1%
Iran, Islamic Republic of 1 1%
Russia 1 1%
United States 1 1%
Unknown 80 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 27%
Student > Master 20 24%
Researcher 12 14%
Professor 5 6%
Student > Bachelor 4 5%
Other 8 9%
Unknown 13 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 21%
Medicine and Dentistry 10 12%
Biochemistry, Genetics and Molecular Biology 9 11%
Chemistry 6 7%
Pharmacology, Toxicology and Pharmaceutical Science 6 7%
Other 18 21%
Unknown 18 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2017.
All research outputs
#5,340,716
of 25,584,565 outputs
Outputs from International Journal of Nanomedicine
#484
of 4,077 outputs
Outputs of similar age
#32,404
of 168,428 outputs
Outputs of similar age from International Journal of Nanomedicine
#12
of 77 outputs
Altmetric has tracked 25,584,565 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,077 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 168,428 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.