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Oxidative DNA damage in lung tissue from patients with COPD is clustered in functionally significant sequences

Overview of attention for article published in International Journal of Chronic Obstructive Pulmonary Disease, March 2011
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

blogs
1 blog
twitter
1 tweeter

Citations

dimensions_citation
47 Dimensions

Readers on

mendeley
22 Mendeley
Title
Oxidative DNA damage in lung tissue from patients with COPD is clustered in functionally significant sequences
Published in
International Journal of Chronic Obstructive Pulmonary Disease, March 2011
DOI 10.2147/copd.s15922
Pubmed ID
Authors

Mark N. Gillespie, Victor M. Pastukh, Zhang, Mykhaylo Ruchko, Olena Gorodnya, Bardwell, Rubin Tuder

Abstract

Lung tissue from COPD patients displays oxidative DNA damage. The present study determined whether oxidative DNA damage was randomly distributed or whether it was localized in specific sequences in either the nuclear or mitochondrial genomes. The DNA damage-specific histone, gamma-H2AX, was detected immunohistochemically in alveolar wall cells in lung tissue from COPD patients but not control subjects. A PCR-based method was used to search for oxidized purine base products in selected 200 bp sequences in promoters and coding regions of the VEGF, TGF-β1, HO-1, Egr1, and β-actin genes while quantitative Southern blot analysis was used to detect oxidative damage to the mitochondrial genome in lung tissue from control subjects and COPD patients. Among the nuclear genes examined, oxidative damage was detected in only 1 sequence in lung tissue from COPD patients: the hypoxic response element (HRE) of the VEGF promoter. The content of VEGF mRNA also was reduced in COPD lung tissue. Mitochondrial DNA content was unaltered in COPD lung tissue, but there was a substantial increase in mitochondrial DNA strand breaks and/or abasic sites. These findings show that oxidative DNA damage in COPD lungs is prominent in the HRE of the VEGF promoter and in the mitochondrial genome and raise the intriguing possibility that genome and sequence-specific oxidative DNA damage could contribute to transcriptional dysregulation and cell fate decisions in COPD.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 27%
Researcher 5 23%
Student > Bachelor 3 14%
Professor > Associate Professor 3 14%
Lecturer > Senior Lecturer 2 9%
Other 3 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 45%
Medicine and Dentistry 6 27%
Biochemistry, Genetics and Molecular Biology 3 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Unknown 2 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 October 2019.
All research outputs
#2,655,761
of 15,968,783 outputs
Outputs from International Journal of Chronic Obstructive Pulmonary Disease
#337
of 1,863 outputs
Outputs of similar age
#20,928
of 128,869 outputs
Outputs of similar age from International Journal of Chronic Obstructive Pulmonary Disease
#6
of 34 outputs
Altmetric has tracked 15,968,783 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,863 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.8. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 128,869 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.