| Title |
Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis
|
|---|---|
| Published in |
International Journal of Nanomedicine, June 2016
|
| DOI | 10.2147/ijn.s103762 |
| Pubmed ID | |
| Authors |
Seda Rençber, Sinem Yaprak Karavana, Fethiye Ferda Yılmaz, Bayri Eraç, Merve Nenni, Seda Özbal, Çetin Pekçetin, Hande Gurer-Orhan, Mine Hoşgör-Limoncu, Pelin Güneri, Gökhan Ertan |
| Abstract |
This study aimed to develop a suitable buccal mucoadhesive nanoparticle (NP) formulation containing fluconazole for the local treatment of oral candidiasis. The suitability of the prepared formulations was assessed by means of particle size (PS), polydispersity index, and zeta potential measurements, morphology analysis, mucoadhesion studies, drug entrapment efficiency (EE), in vitro drug release, and stability studies. Based on the optimum NP formulation, ex vivo drug diffusion and in vitro cytotoxicity studies were performed. Besides, evaluation of the antifungal effect of the optimum formulation was evaluated using agar diffusion method, fungicidal activity-related in vitro release study, and time-dependent fungicidal activity. The effect of the optimum NP formulation on the healing of oral candidiasis was investigated in an animal model, which was employed for the first time in this study. The zeta potential, mucoadhesion, and in vitro drug release studies of various NP formulations revealed that chitosan-coated NP formulation containing EUDRAGIT(®) RS 2.5% had superior properties than other formulations. Concerning the stability study of the selected formulation, the formulation was found to be stable for 6 months. During the ex vivo drug diffusion study, no drug was found in receptor phase, and this is an indication of local effect. The in vitro antifungal activity studies showed the in vitro efficacy of the NP against Candida albicans for an extended period. Also, the formulation had no cytotoxic effect at the tested concentration. For the in vivo experiments, infected rabbits were successfully treated with local administration of the optimum NP formulation once a day. This study has shown that the mucoadhesive NP formulation containing fluconazole is a promising candidate with once-a-day application for the local treatment of oral candidiasis. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | <1% |
| Unknown | 113 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 23 | 20% |
| Researcher | 12 | 11% |
| Student > Ph. D. Student | 12 | 11% |
| Student > Bachelor | 12 | 11% |
| Professor > Associate Professor | 5 | 4% |
| Other | 18 | 16% |
| Unknown | 32 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 28 | 25% |
| Medicine and Dentistry | 15 | 13% |
| Biochemistry, Genetics and Molecular Biology | 8 | 7% |
| Chemistry | 4 | 4% |
| Nursing and Health Professions | 3 | 3% |
| Other | 15 | 13% |
| Unknown | 41 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2016.
All research outputs
#17,302,400
of 25,394,764 outputs
Outputs from International Journal of Nanomedicine
#2,472
of 4,122 outputs
Outputs of similar age
#225,429
of 353,760 outputs
Outputs of similar age from International Journal of Nanomedicine
#98
of 123 outputs
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So far Altmetric has tracked 4,122 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
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