| Title |
Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
|
|---|---|
| Published in |
Clinical Ophthalmology, June 2016
|
| DOI | 10.2147/opth.s103443 |
| Pubmed ID | |
| Authors |
Jong Hwa Jun, Wern-Joo Sohn, Youngkyun Lee, Jae-Young Kim |
| Abstract |
The molecular and cellular effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells (LECs) were examined using both an immortalized human lens epithelial cell line and a porcine capsular bag model. After treatment with various concentrations of bevacizumab, cell viability and proliferation patterns were evaluated using the water-soluble tetrazolium salt assay and 5-bromo-2'-deoxyuridine enzyme-linked immunosorbent assay, respectively. The scratch assay and Western blot analysis were employed to validate the cell migration pattern and altered expression levels of signaling molecules related to the epithelial-mesenchymal transition (EMT). Application of bevacizumab induced a range of altered cellular events in a concentration-dependent manner. A 0.1-2 mg/mL concentration demonstrated dose-dependent increase in proliferation and viability of LECs. However, 4 mg/mL decreased cell proliferation and viability. Cell migrations displayed dose-dependent retardation from 0.1 mg/mL bevacizumab treatment. Transforming growth factor-β2 expression was markedly increased in a dose-dependent manner, and α-smooth muscle actin, matrix metalloproteinase-9, and vimentin expression levels showed dose-dependent changes in a B3 cell line. Microscopic observation of porcine capsular bag revealed changes in cellular morphology and a decline in cell density compared to the control after 2 mg/mL treatment. The central aspect of posterior capsule showed delayed confluence, and the factors related to EMT revealed similar expression patterns to those identified in the cell line. Based on these results, bevacizumab modulates the proliferation and viability of LECs and induces morphological alterations through the modulation of expression patterns of specific factors related to the EMT. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 11% |
| Unknown | 8 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 22% |
| Student > Ph. D. Student | 2 | 22% |
| Lecturer > Senior Lecturer | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Other | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Agricultural and Biological Sciences | 2 | 22% |
| Medicine and Dentistry | 2 | 22% |
| Nursing and Health Professions | 1 | 11% |
| Design | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 July 2016.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Clinical Ophthalmology
#3,207
of 3,712 outputs
Outputs of similar age
#309,346
of 353,659 outputs
Outputs of similar age from Clinical Ophthalmology
#83
of 85 outputs
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